Mechanisms of DNA break processing for homologous recombination
Molecular Horizons Seminar - Professor Petr Cejka
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Online via Zoom
My laboratory is using biochemical tools to understand mechanisms of DNA double-strand break repair by homologous recombination. In the first part of the seminar, I will talk about how the breast cancer suppressor BRCA1 promotes the initial processing (resection) of DNA breaks. I will show that BRCA1 directly promotes long-range resection by BLM/DNA2 in conjunction with CtIP. In the second part of my talk, I will show that DNA breaks formed by Cas9 cannot be immediately processed because Cas9 bridges the broken DNA together. I will show that HLTF, a dsDNA translocase, can efficiently remove Cas9 from DNA, with potential implications for gene editing.